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purified gsps  (Kikkoman Corporation)

 
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    Kikkoman Corporation purified gsps
    (A) <t>GSPs</t> prevent transferable suppression by UVB through the inactivation of UVB-induced CD4+ suppresser T cells. Mice were treated as described in Figure 3 and CD4+ T <t>cells</t> <t>purified</t> from the splenocytes and lymphocytes by positive selection. Twenty-four hours after i.v. injection of CD4+ T cells into naïve mice, the mice were sensitized with DNFB, and ear challenged 5 days after sensitization, as detailed in the Materials and Methods. Those naïve mice that received CD4+ T cells from UVB-exposed donor mice that were GSPs treated showed a greater CHS response than UVB-exposed mice that were not GSPs treated. Those naïve mice that received CD4+ T cells from UVB-exposed rIL-12-injected donor mice showed a significantly higher CHS response than UVB-exposed mice that were not injected with rIL-12. The change in the ear swelling response in each group is reported as mean ± SD (n=5 per group). Significantly greater CHS response versus UVB irradiation in the absence of GSPS or rIL-12 treatment, †P<0.001; Significantly lower CHS response versus positive control group (2nd bar), ¶P<0.01. (B) Treatment of mice with GSPs or rIL-12 decreases the production of IL-4 and IL-10 by CD4+ T cells. Mice were sensitized to DNFB after UVB-irradiation described in Figure 3, the CD4+ T cells isolated by positive selection and then co-cultured with DNSB-labeled BMDC for 48 h, as detailed in the Materials and Methods. The concentrations of cytokines in the cell supernatants were estimated by ELISA and are presented as the mean ±SD in terms of pg/2 million cells. Experiment was repeated once, n=5. Significant decrease versus UVB+DNFB group, †P<0.001; Significant decrease versus positive control (DNFB alone), ¶P<0.001; Significant increase versus positive control (DNFB alone), *P<0.001
    Purified Gsps, supplied by Kikkoman Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/purified+gsps/purified+gsps/pmc03033965-103-2-7
    Average 90 stars, based on 1 article reviews
    purified gsps - by Bioz Stars, 2026-10
    90/100 stars

    Images

    1) Product Images from "Proanthocyanidins Inhibit UV-Induced Immunosuppression Through IL-12-Dependent Stimulation of CD8 + Effector T Cells and Inactivation of CD4 + T Cells"

    Article Title: Proanthocyanidins Inhibit UV-Induced Immunosuppression Through IL-12-Dependent Stimulation of CD8 + Effector T Cells and Inactivation of CD4 + T Cells

    Journal:

    doi: 10.1158/1940-6207.CAPR-10-0224

    (A) GSPs prevent transferable suppression by UVB through the inactivation of UVB-induced CD4+ suppresser T cells. Mice were treated as described in Figure 3 and CD4+ T cells purified from the splenocytes and lymphocytes by positive selection. Twenty-four hours after i.v. injection of CD4+ T cells into naïve mice, the mice were sensitized with DNFB, and ear challenged 5 days after sensitization, as detailed in the Materials and Methods. Those naïve mice that received CD4+ T cells from UVB-exposed donor mice that were GSPs treated showed a greater CHS response than UVB-exposed mice that were not GSPs treated. Those naïve mice that received CD4+ T cells from UVB-exposed rIL-12-injected donor mice showed a significantly higher CHS response than UVB-exposed mice that were not injected with rIL-12. The change in the ear swelling response in each group is reported as mean ± SD (n=5 per group). Significantly greater CHS response versus UVB irradiation in the absence of GSPS or rIL-12 treatment, †P<0.001; Significantly lower CHS response versus positive control group (2nd bar), ¶P<0.01. (B) Treatment of mice with GSPs or rIL-12 decreases the production of IL-4 and IL-10 by CD4+ T cells. Mice were sensitized to DNFB after UVB-irradiation described in Figure 3, the CD4+ T cells isolated by positive selection and then co-cultured with DNSB-labeled BMDC for 48 h, as detailed in the Materials and Methods. The concentrations of cytokines in the cell supernatants were estimated by ELISA and are presented as the mean ±SD in terms of pg/2 million cells. Experiment was repeated once, n=5. Significant decrease versus UVB+DNFB group, †P<0.001; Significant decrease versus positive control (DNFB alone), ¶P<0.001; Significant increase versus positive control (DNFB alone), *P<0.001
    Figure Legend Snippet: (A) GSPs prevent transferable suppression by UVB through the inactivation of UVB-induced CD4+ suppresser T cells. Mice were treated as described in Figure 3 and CD4+ T cells purified from the splenocytes and lymphocytes by positive selection. Twenty-four hours after i.v. injection of CD4+ T cells into naïve mice, the mice were sensitized with DNFB, and ear challenged 5 days after sensitization, as detailed in the Materials and Methods. Those naïve mice that received CD4+ T cells from UVB-exposed donor mice that were GSPs treated showed a greater CHS response than UVB-exposed mice that were not GSPs treated. Those naïve mice that received CD4+ T cells from UVB-exposed rIL-12-injected donor mice showed a significantly higher CHS response than UVB-exposed mice that were not injected with rIL-12. The change in the ear swelling response in each group is reported as mean ± SD (n=5 per group). Significantly greater CHS response versus UVB irradiation in the absence of GSPS or rIL-12 treatment, †P<0.001; Significantly lower CHS response versus positive control group (2nd bar), ¶P<0.01. (B) Treatment of mice with GSPs or rIL-12 decreases the production of IL-4 and IL-10 by CD4+ T cells. Mice were sensitized to DNFB after UVB-irradiation described in Figure 3, the CD4+ T cells isolated by positive selection and then co-cultured with DNSB-labeled BMDC for 48 h, as detailed in the Materials and Methods. The concentrations of cytokines in the cell supernatants were estimated by ELISA and are presented as the mean ±SD in terms of pg/2 million cells. Experiment was repeated once, n=5. Significant decrease versus UVB+DNFB group, †P<0.001; Significant decrease versus positive control (DNFB alone), ¶P<0.001; Significant increase versus positive control (DNFB alone), *P<0.001

    Techniques Used: Purification, Selection, Injection, Irradiation, Positive Control, Isolation, Cell Culture, Labeling, Enzyme-linked Immunosorbent Assay



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    Image Search Results


    (A) GSPs prevent transferable suppression by UVB through the inactivation of UVB-induced CD4+ suppresser T cells. Mice were treated as described in Figure 3 and CD4+ T cells purified from the splenocytes and lymphocytes by positive selection. Twenty-four hours after i.v. injection of CD4+ T cells into naïve mice, the mice were sensitized with DNFB, and ear challenged 5 days after sensitization, as detailed in the Materials and Methods. Those naïve mice that received CD4+ T cells from UVB-exposed donor mice that were GSPs treated showed a greater CHS response than UVB-exposed mice that were not GSPs treated. Those naïve mice that received CD4+ T cells from UVB-exposed rIL-12-injected donor mice showed a significantly higher CHS response than UVB-exposed mice that were not injected with rIL-12. The change in the ear swelling response in each group is reported as mean ± SD (n=5 per group). Significantly greater CHS response versus UVB irradiation in the absence of GSPS or rIL-12 treatment, †P<0.001; Significantly lower CHS response versus positive control group (2nd bar), ¶P<0.01. (B) Treatment of mice with GSPs or rIL-12 decreases the production of IL-4 and IL-10 by CD4+ T cells. Mice were sensitized to DNFB after UVB-irradiation described in Figure 3, the CD4+ T cells isolated by positive selection and then co-cultured with DNSB-labeled BMDC for 48 h, as detailed in the Materials and Methods. The concentrations of cytokines in the cell supernatants were estimated by ELISA and are presented as the mean ±SD in terms of pg/2 million cells. Experiment was repeated once, n=5. Significant decrease versus UVB+DNFB group, †P<0.001; Significant decrease versus positive control (DNFB alone), ¶P<0.001; Significant increase versus positive control (DNFB alone), *P<0.001

    Journal:

    Article Title: Proanthocyanidins Inhibit UV-Induced Immunosuppression Through IL-12-Dependent Stimulation of CD8 + Effector T Cells and Inactivation of CD4 + T Cells

    doi: 10.1158/1940-6207.CAPR-10-0224

    Figure Lengend Snippet: (A) GSPs prevent transferable suppression by UVB through the inactivation of UVB-induced CD4+ suppresser T cells. Mice were treated as described in Figure 3 and CD4+ T cells purified from the splenocytes and lymphocytes by positive selection. Twenty-four hours after i.v. injection of CD4+ T cells into naïve mice, the mice were sensitized with DNFB, and ear challenged 5 days after sensitization, as detailed in the Materials and Methods. Those naïve mice that received CD4+ T cells from UVB-exposed donor mice that were GSPs treated showed a greater CHS response than UVB-exposed mice that were not GSPs treated. Those naïve mice that received CD4+ T cells from UVB-exposed rIL-12-injected donor mice showed a significantly higher CHS response than UVB-exposed mice that were not injected with rIL-12. The change in the ear swelling response in each group is reported as mean ± SD (n=5 per group). Significantly greater CHS response versus UVB irradiation in the absence of GSPS or rIL-12 treatment, †P<0.001; Significantly lower CHS response versus positive control group (2nd bar), ¶P<0.01. (B) Treatment of mice with GSPs or rIL-12 decreases the production of IL-4 and IL-10 by CD4+ T cells. Mice were sensitized to DNFB after UVB-irradiation described in Figure 3, the CD4+ T cells isolated by positive selection and then co-cultured with DNSB-labeled BMDC for 48 h, as detailed in the Materials and Methods. The concentrations of cytokines in the cell supernatants were estimated by ELISA and are presented as the mean ±SD in terms of pg/2 million cells. Experiment was repeated once, n=5. Significant decrease versus UVB+DNFB group, †P<0.001; Significant decrease versus positive control (DNFB alone), ¶P<0.001; Significant increase versus positive control (DNFB alone), *P<0.001

    Article Snippet: The purified GSPs were obtained from the Kikkoman Corporation (Tokyo, Japan) and the chemical composition has been described earlier ( 17 , 18 ).

    Techniques: Purification, Selection, Injection, Irradiation, Positive Control, Isolation, Cell Culture, Labeling, Enzyme-linked Immunosorbent Assay